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After approximately 6 mo without access to a deprivatioj female, eight old 19 to 25 yr and eight middle-aged 9 to 15 yr males were given nine daily min tests of sexual behavior with receptive females.

Three ovariectomized, estrogen-primed females served as partners. Blood samples were taken from the males at and h on 2 consecutive days before and after the series of nine behavioral tests.

Serum levels of testosterone T effechs measured by means of radioimmunoassay. There were no significant differences between serum T levels after 6 mo of sexual deprivation and after the nine tests of sexual behavior, nor did serum T levels differ between middle-aged and old males.

Serum T levels were significantly higher in the h samples than in the h samples for both groups. The nocturnal increases in serum T, both absolute amounts and percentages of increase, did not differ between middle-aged and old males.

Repeated daily testing had little effect on the major components of male sexual behavior. Middle-aged males outperformed old males; middle-aged males efects higher rates of contracting the female, mounting and intromitting.

They ejaculated earlier and had a greater percentage of tests with ejaculations than did old males.